Rubric – Microbiology College/University Regulation of the tryptophan operon

Criteria / Topics 0-40 Points
TrpR – The trp repressor
  • 0 points: No mention of TrpR or incorrect description of its behavior.
  • 20 points: TrpR is mentioned, and its behavior is correctly described (synthesized in an inactive state, activated by tryptophan binding, binds to operator site and blocks transcription).
Attenuation
  • 0 points: No mention of attenuation or incorrect explanation of its mechanism.
  • 20 points: Attenuation is mentioned, and its mechanism is correctly explained (leader peptide, translation, tryptophan codons, 3:4 loop, terminator, 2:3 loop).
Feedback inhibition
  • 0 points: No mention of feedback inhibition or incorrect explanation of its effect on anthranilate synthase.
  • 20 points: Feedback inhibition is mentioned, and its effect on anthranilate synthase is correctly explained (tryptophan binding to allosteric site, decrease in activity, shutting down the pathway).
Other mistakes
  • 0 points: Other forms of regulation other than TrpR, attenuation, or feedback inhibition are mentioned.
  • 20 points: No other forms of regulation mentioned.
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